This was a great week and a not so great week. Elisa was able to use the protein we purified to run a 1D HSQC NMR experiment of our protein. From the spectra we can tell that there is nothing wrong with our protein it is well folded. Elisa one of our lab mentors, has a sample of very precious protein that she needed to screen, and was just sitting in 4℃. Jose and I were going to learn how to set up the sample for NMR analysis, unfortunately, one of the NMR machines was not working. The second machine was working there were some issues with the sample changer but Elisa said we could do this manually. Then, sadly there were some issues with the sample changer and Elisa could not start the experiments over the weekend.
In regards to protein expression, Jose and I started to express the second protein of interest. Unfortunately, we did not get good purification or yield. We are going to give it a second attempt. Along with the laboratory work, we have also been learning background information necessary for NMR use, and understanding. Elisa is great mentor and does a great job simplifying the concepts for us.
I really enjoyed learning about all of your advancements and struggles in your respective experiments. It is great to hear about what we all do. I wish you all great luck in your experiments and I am excited to hear more about what your findings are. Wish you all have a great week J .
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