Sanford Burnham Medical Research Institute
Sunday, July 7, 2013
Week 2
Another week has past us by at Sanford Burnham and I have to say I am really becoming comfortable with our project, which deals with the protein Bid and EphA3. Our goal is to identify and possibly synthesize peptide(s) that bind to Bid. We plan to do this by first purifying and expressing the protein, screening the library of compounds by NMR, synthesizing the individual peptides, and finally characterizing the peptides. Through this point, we have been detailing our course of action, mapping out our deadlines for our eight weeks here, intensively researching and gaining more background knowledge for our project and formulating our presentation for this upcoming week. Since our post-doc mentor, Elisa is on vacation, we have been able to interact with Dr. Pellechia more on a daily basis. He has clearly mapped out and explained his expectations and has also been a big help in regards to our presentation by giving us pointers and constructive criticism. I am actually pretty excited to present! This upcoming week is when the real fun starts as we will be commencing our protein expression of Bid!
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This second week was a short week due to the 4th of July, but it was also a very busy week. This was also the week that Claudia and I got to work on our own, I was both exited and nervous. The main focus of this week was enzyme kinetics in which we measured the rate of product formation using different enzyme and substrate combinations. Although I am not an expert on Enzyme kinetics it feels good to get the expected reaction rates. My next goal is to learn to interpret the information that we acquired and apply to the next step in our lab assignment.
ReplyDeleteFor the second week of the internship we met with Dr. Kwon on Tuesday July 2nd to go over the literature both Griseld and I had found regarding our topics. My first idea was to focus on whether obesity and pesticides were risk factors for prostate cancer. After meeting with Dr. Kwon he suggest we began narrowing down our literature to more specific topics and more narrowed findings. Thus I began to do more research on the background and definition of Obesity. Dr. Kwon also suggested that we began to look at data sets contained on both the CHIS and SEER data base. Hence, I began looking for different data sets that would help support my ideas. We we advised to begin making a general outline of our project, which included a main topic (developing hypothesis), questions, and finding data to support.
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