Yay! The NMR was finally fixed, Jose and I had the opportunity to shadow Elisa during both the sample prep and the NMR screening. The only downside was that the sample changer was still not working and Elisa had to go and manually change the sample every 30 min.
It was an incredible experience to be inside an NMR facility. These machines are huge. It is not a great scenery but the excitement comes from knowing how powerful this piece of equipment is. It has the capability of giving you information bits of the chemical components to build the map of protein and identify what shifts the peptide has generated by binding to the protein.
Most of our week was centered in protein expression and purification and NMR specrtra analysis. Jose and I scanned all of the 76 spectras in four days. More detail about this and the steps to follow will be provided in the presentation this week. At the end of week 6 we had the fantastic news that the sample changer was fixed and is know working beautifully.
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