Week 5

     In week 5 I enjoyed learning how to do Dot Blots, especially how we were able to adapt the protocol to use the technique with the probes of interests. Although we had some setbacks with some of our Dot Blots not obtaining what we expected, I was able to learn a new tool and how to adapt a protocol and get the expected result. This week we also did kinetics with PR3 and substrate four. It was observed that this enzyme is not soluble. It was interesting to see the difference between a non-soluble enzyme and a soluble one. In addition I was very glad we were able to get results for the presentation and I agree with Philip, this round of presentations went smother. I was able to apply some presentation tips Dr. Salvesen gave to Luis and me, which was very helpful.