Week 6

     This week, we returned to a sense of normalcy. Judith helped us immensely, and we ran western blots, a couple PCRs, and polysomes. Since we didnt get any results from our western blots last week (we now realize it was due to technique error), we carefully reran the ssa2 (24hr on, 1 hr recover) blot. the images we developed were good and conclusive. Unfortunately, we flubbed the transfer for the sks2 membranes since no one set a timer. We will rerun the blot on Monday.
     The polysomes were not conclusive. We used the 24hr on +5hr recovery ( switching from EMM to YES media) samples. The profiles did not reveal a rescue effect, so we will run western blots to see if there was enough of the protein expressed.

This Friday, we went out for dinner. Pizza on Pearl is an Italian restaurant that I had previously visited. We split a pizza which was a welcome break from cooking. On Saturday, I went exploring in the area surrounding the campus. We had some old bread, so I was looking for some seagulls (and birds really) to feed. I must have walked about 4 miles looking for some birds. I went all the way out to the glider port but decided that the 2 mile hike down to the beach was a bit too far just to feed birds. When I came back, I walked around the Eleanor Roosevelt College (ERC)  campus and found a bird by a dumpster. After feeding it a piece, it decided to fly off. Instead of chasing after the bird, I left the remaining bread on the ground by a dumpster, so the bird could return and eat it.

This weekend has been particularly windy, and there have been numerous people flying in gliders and hot air balloons. There is a great view of both from the apartment.


That's all for now. Im looking forward to the presentations this Friday. I will check-in in a week's time.