Week 6

This was a very busy week, we repeated many Dot Blots. We troubleshoot by changing the protein and probe concentrations and we still were not happy with our results. We had a spotty background, or fluorescence where we did not want to have a signal. Finally we left for the weekend with a good Dot Blot that had the signal where there needed to be one for HNE. However we still saw no binding of probe in PR3 :( in a Dot Blot, but there was PR3 and probe binding in a Western Blot.